Monday, October 27, 2008

So I've been away...

So I haven't posted in a while because, well, I hate everything.

Work in the rotation lab is not going well. On two separate occasions, my cells have become contaminated with yeast. I have no idea how this is even possible. I have been doing tissue culture work for years, and I have never had a problem. Now suddenly this has happened twice. Twice! The PI looks at me like I'm an idiot.

I finally got enough cells growing to do a immunoprecipitation experiment. I did the IP just fine, but today when I went to run the gel all hell broke loose. Someone prepared the running buffer wrong, so although the current was steady - there were 700 volts flowing through the damn gel. The cassette got so hot that it cracked the glass and my gel dribbled out of the casing, and I lost my sample. It took me forever to get that shit ready for the experiment and a lot of time doing the experiment itself, only to be foiled by a buffer. What. The. Fuck.

I'm bringing contamination and destruction everywhere I go. It's like I'm living in bizarro world and I'm completely incompetent.

I can't wait to get back to my real lab.

Class is still horrible. I'm still doing really bad on exams for some unknown reason, even though the material isn't hard. I don't get this, either.

I went to a conference a few weeks ago. I was all prepared for people to ignore my poster, as is usually the case - but this time was different. I had quite a few people show up to talk to me during the poster session. In addition, I heard from my work colleague that he saw about four or five people taking photos of my poster when the hall was empty.

I hate people that do this kinda shit. I mean, if you aren't able to come up with your own ideas and your own experimental design, why the fuck are you in science at all? If you need to photograph the slides from someone's talk because you can't figure shit out on your own, you should turn in your degree. I absolutely can't stand these assholes.

Now I have to start writing my paper so I don't get scooped. I don't know what I will do if I get scooped. I'm pretty sure that petrol bombing would fit in there somewhere.

Man, I thought grad school was going to be great. I'm being challenged in all the wrong ways, though. Pointless memorization. Idea theft. Dickbag professors. And worst of all, the stifling of my creativity. I'm sure I can use all of these lessons eventually, but that fact is really not making things any better. This sucks.

So yeah, I hate everything.

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